RNA-Seq is a popular method for measuring differential gene expression. This hands-on workshop introduces the concepts of RNA-Seq analysis, from data preparation through to statistical testing for differential gene expression. You’ll learn how to use popular tools and workflows to analyse the data, produce graphical summaries and identify differentially expressed genes.

The workshop will focus on the use of Galaxy, a web-based platform for accessible, reproducible, and transparent computational biological research. Widely used by researchers world wide, Galaxy gives you access to 1000’s of popular tools for analysis and processing of biological data. Galaxy Australia is a fully subsidised service provided by Australian BioCommons. 

Learning outcomes:

By the end of the workshop you should be able to:

• Perform QC evaluation and filtering on next-generation sequencing data

• Use tools in Galaxy to identify differentially expressed genes from RNA-Seq data

Format:

This online workshop will take place over two four-hour sessions. You must attend both sessions in order to get the most out of the workshop. Expert trainers will introduce new topics and guide you through hands-on activities to help you put your new skills into action.

Lead Trainer:

Mike Thang, Galaxy Australia
Date/Time:

10 & 11 September 2024, 1 - 5 pm AEST/12:30 - 4:30 pm ACST/11 am - 3 pm AWST 

Who the workshop is for:

This workshop is for Australian researchers and life scientists who will use RNA-seq for differential gene expression as part of their projects. 

Prerequisites

• You must be associated with an Australian organisation for your application to be considered.

• Some familiarity with Galaxy is encouraged. We recommend watching this brief introduction to Galaxy Australia and working through the Galaxy Basics for Everyone tutorial before joining the workshop. Alternatively you can join our introductory workshop on 21 August.

How to apply:

This workshop is free but participation is subject to application with selection. 

Applications have closed.

Applications will be reviewed by the organising committee and all applicants will be informed of the status of their application (successful, waiting list, unsuccessful). Successful applicants will be provided with a Zoom meeting link closer to the date. More information on the selection process is provided in our Advice on applying for Australian BioCommons workshops.

This workshop is presented by the Australian BioCommons and QCIF with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.

This event is part of a series of bioinformatics training events. If you'd like to hear when registrations open for other events, please subscribe to the Australian BioCommons newsletter.

Cover image: Heatmap of differentially expressed genes from Corley et al (2016), BMC Genomics. Reproduced under the terms of the Creative Commons Attribution 4.0 International License.