An introduction to RNA-seq analysis using the Galaxy Australia web platform
About this workshop
RNA-seq is a popular method for simultaneously measuring and comparing the expression of tens of thousands of genes in multiple samples.
There are many steps involved in analysing RNA-Seq data. The analysis begins with sequencing reads (FASTQ files). These are usually aligned to a reference genome. The number of reads mapped to each gene can then be counted. This results in a table of counts, which is what we perform statistical analyses on to determine differentially expressed genes and pathways.
This practical workshop provides a hands-on introduction to RNA-seq analysis from reads to counts, to differential expression. The workshop makes use of Galaxy, an online platform for biological research that allows people to use computational data analysis tools and workflows without the need for programming experience.
The workshop materials are based on the following Galaxy training workshops:
Learning objectives
By the end of the workshop you should be able to:
Perform quality control of short reads using FastQC
Map reads to a reference genome using HISAT
Determine the number of reads mapped across genes or exons
Normalise read counts to account for composition bias
Perform differential expression analysis
Visualise results using a variety of plots
Who the workshop is for
Researchers who have, or will soon acquire, RNA-seq short read data that they need to analyse themselves. No prior experience with RNA-seq analysis is required.
The workshop requires you to be familiar with using and navigating Galaxy Australia.
If you are new to the Galaxy platform (an online platform for computational analysis) we recommend that you work through the Galaxy 101 For Everyone tutorial in advance of the workshop.
Lead trainers: Ms Jessica Chung, Dr Tom Harrop
Date/Time: 23 - 24 February 2:00 - 5:00 pm AEDT
How to apply
The workshop is free but due to anticipated high demand, participation is subject to application with selection. Places are strictly limited to 30 participants.
Ineligible or incomplete applications will not be considered. For your application to be considered you must:
Have an Australian institutional email address
Have, or will soon acquire, RNA-seq data
Complete the application form
Applications will be reviewed by the organising committee and all applicants will be informed of the status of their application (successful, waiting list, unsuccessful) by Tuesday 16 February 2021.
Please complete the application form if you are interested in attending this workshop. Applications close 5pm AEDT Friday 5 February 2021.
The workshop is presented by the Australian Biocommons and Melbourne Bioinformatics with the assistance of a network of Facilitators from around Australia.
This event is part of a series of bioinformatics training events. If you'd like to hear when registrations open for other events, please subscribe to Australian Biocommons.
Cover image credit: Thomas Shafee
